摘要
目的 确定兔抗NMDAr1亚基 (N 甲基 D 天冬氨酸受体Nr1亚基 )IgG分子特征性构象。方法 应用原子力显微镜扫描生理状态下分布在云母表面的兔抗NMDAr1亚基蛋白IgG分子 ,并进行理论计算。结果 IgG分子为 136 .4 ×6 2 .8 × 2 6 .1 椭球形三亚基复合物。结论 原子力显微镜可以在生理状态下直观测定生物大分子纳米尺度介观结构。抗NMDAr1蛋白IgG分子的特征性构象 ,可以做为神经细胞膜表面NMDA受体 (N 甲基 D 天冬氨酸受体 )
Objective To investigate whether IgG could be used as a label when binded with antigen of membrane protein ,and study the characteristics of conformation of anti-NMDAr1 polycolonal IgG. Methods AFM was used to detect images of anti-NMDAr1 polycolonal IgG affiliated on mica under physiological environment in order to determine the characteristic 3D structure of it.Results The images and data showed that the anti-NMDAr1 polycolonal IgG has characteristic structure described as an elliptical sphere of 136.4?× 62.8?×26.1?.On section, there were two peaks with the width of 13 nm at the edge of ellipse. Conclusion Atomic force microscopy is a new method which is capable to provide images at a nanometer scale resolution. It could be used to study the structure of IgG.IgG could be used as a label in the study of membrane receptor protein.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2004年第3期188-191,共4页
Immunological Journal
基金
国家自然科学基金 (30 1 70 961 )
军队十五重点科研基金(0 1Z0 54)资助
