摘要
目的 探讨去甲肾上腺素 (norepinephrine ,NE)刺激心肌细胞内活性氧 (reactiveoxygenspecies ,ROS)产生的量效、时效关系及受体途径。方法 用DCFH DA荧光探针标记培养乳鼠心肌细胞 ,流式细胞仪检测细胞荧光强度 (反映ROS水平 ) ,观察不同浓度和不同时相的NE刺激对心肌细胞ROS生成影响及普萘洛尔和哌唑嗪的干预效果。结果 NE呈剂量依赖性及时间依赖性增加心肌细胞内ROS的产生 ,哌唑嗪或普萘洛尔均能部分抑制NE的促ROS生成的作用 ,而哌唑嗪和普萘洛尔共同干预 ,则几乎可完全抑制NE介导的心肌细胞内ROS产生。结论 NE通过α 肾上腺素能受体和 β 肾上腺素能受体介导 ,以剂量依赖性及时间依赖性的方式刺激心肌细胞内ROS的生成。
Objective To study the effects of norepinephrine (NE) on intracellular reactive oxygen species (ROS) production in cardiac myocytes and receptor pathways involved in ROS production. Methods DCFHDA fluorescent probe was used for the assessment of NE induced intracellular ROS production in cultured cardiac myocytes of the neonatal rats. Myocytes were exposed to NE, NE + prazosin, NE + propranolol, and NE + prazosin + propranolol at different time and doses. The changes of fluorescence intensity in cells were measured by flow cytometry. Results NE increased myocyte ROS production in time and dose dependent manners. NE induced ROS production was partially inhibited by prazosin or propranolol, and completely inhibited by prazosin and propranolol together in myocytes. Conclusion NE can induce intracellular ROS production in cultured cardiac myocytes of the neonatal rats in time and dose dependent manners through the mediation of both α and β adrenoceptors.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2004年第8期694-696,共3页
Journal of Third Military Medical University
关键词
去甲肾上腺素
心肌细胞
活性氧
norepinephrine
myocardiocyte
reactive oxygen species
