摘要
利用PLO(Poly-L-Ornithine)将外源PLRV-CP基因导入到马铃薯加工型品种大西洋原生质体中,获得了转化后代,经过PCR和RT-PCR分析均显阳性,蚜虫接种PLRV,结果比对照明显抗PLRV。
The external PLRV-CP gene was transferred into processing Potato protoplasts and got transferred progeny by PLO. The PCR and RT-PCR analysis was positive. After inoculating PLRV with aphid, the transplant was much more resistant to PLRV than control.
出处
《黑龙江农业科学》
2004年第3期1-3,共3页
Heilongjiang Agricultural Sciences
基金
黑龙江省科委攻关资助项目(GA01B101-08)