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大鼠松果体钟基因Clock的昼夜节律性表达 被引量：1

Circadian Expression of the Clock Gene in the Rat Pineal Gland
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摘要 探讨 12h光照、12h黑暗交替 (LD)光制下松果体钟基因Clock是否存在昼夜节律性表达。在LD光制下饲养SD大鼠 4周后 ,在一昼夜内每隔 4h采集松果体组织 ,提取总RNA ,进行竞争性定量RT PCR ,测定不同昼夜时点(CT)样品中ClockmRNA的相对表达量。用余弦函数获取节律参数 ,并经振幅检验分析是否存在昼夜节律。结果是松果体Clock基因mRNA表达呈现昼夜节律性振荡变化 (P <0 .0 5 ) ,峰值和谷值分别位于CT18和CT6 ,峰值相位- 2 6 8.76± 2 7.6 3,振幅 0 .4 2± 0 .14 ,中值 0 .99± 0 .10。 The circadian expression of the Clock gene in the rat pineal gland was investigated under the 12 h-light and 12 h-dark cycle condition (LD). 36 SD rats housed under the light regime of LD for four weeks were sampled for the pineal gland every 4 hours in a circadian day. The total RNA was extracted from the samples and the semiquantitative reverse transcription polymerase chain reaction (RT-PCR) was used to determine the temporal changes in mRNA level of the Clock gene during different circadian times (CT). The data parameters were analysed by both the cosine function software and the amplitude F test to reveal the circadian rhythm. The Clock mRNA level in the pineal gland showed a significantly circadian oscillation (P<0.05), with the peak at CT18 and trough at CT6. The parameter values of the peak phase, amplitude and mesor were -268.76±27.63, 0.42±0.14 and 0.99±0.10, respectively. It is concluded that the fingdings demonstrate that the circadian expression of Clock gene in the pineal gland is weak during the daytime and robust at nighttime under LD condition.
作者 王国卿 杜玉珍 童建
机构地区 苏州大学医学院基础医学系生理学教研室 苏州大学放射医学与公共卫生学院卫生毒理学教研室
出处 《科学技术与工程》 2004年第4期262-265,共4页 Science Technology and Engineering
基金 国家自然科学基金 ( 3 0 170 2 95 )资助
关键词 CLOCK基因 昼夜节律 松果体 生物钟 Clock gene circadian rhythm pineal gland
分类号 R322.55 [医药卫生—人体解剖和组织胚胎学] R338.6 [医药卫生—人体生理学]
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参考文献9

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同被引文献8

  • 1[1]Stehle J H, Von Gall C, Korf H W. Melatonin: a clock output,a clock-input. J Neuroendocrin, 2003; 15(4): 383-389
  • 2[3]Klein D C, Coon S L, Roseboom P H, Weller J L, Bernard M,Gastel J A, Zatz M, Iuvone P M, Rodriguez I R, Begay V, Falcon J, Cahill G M, Cassone V M, Baler R. The melatonir rhythm-generating enzyme: molecular regulation of serotonin Nacetyltransferase in the pineal gland. Recent Prog Horm Res,1997: 52: 307-358
  • 3[5]Futscher B W, Flake L L, Gerlach J H, Grogan T M, Dalton W S. Quantitative polymerase chain reaction analysis of mdrl mRNA in multiple myeloma cell lines and clinical specimens. J Analytical Biochem, 1993; 213(2): 414-421
  • 4[6]Fukada Y, Okano T. Circadian clock system in the pineal gland.Mol Neurobiol, 2002; 25(1): 19-30
  • 5[7]Takekida S, Yan L, Maywood E S, Hastings M H, Okamura H. Differential adrenergic regulation of the circadian expression of the clock genes Period 1 and Period 2 in the rat pineal gland.Eur J Neurosci, 2000; 12(12): 4557-4561
  • 6[8]Fukuhara C, Dirden J C, Tosini G. Circadian expression of peri od 1, period 2, and Arylalkylamine N-acetyltransferase mRNA in the rat pineal gland under different light conditions. Neurosci Lett, 2000; 286(3): 167-170
  • 7[9]Hamoui S, Benedetto J P, Garret M, Bonnet J. Quantitation of mRNA species by RT PCR on total mRNA population. PCR Methods ＆ Applications, 1994; 4(3): 160-166
  • 8[10]Namihira M, Honma S, Abe H, Tanahashi Y, Ikeda M, Honma K. Daily variation and light responsiveness of mammalian clock gene, Clock and BMAL1, transcripts in the pineal body and different areas of brain in rats. Neurosci Lett, 1999; 267(1): 69-72

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科学技术与工程
2004年 第4期

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