摘要
目的 探讨血PDGF BB在共育体系中抑制破骨细胞 (OC)功能的机制。方法 将PDGF BB施加于两种培养体系。测定培养上清一氧化氮 (NO)产物的浓度 ;检测诱导型和内皮型一氧化氮合酶表达情况 ;检测共育体系内外骨保护素 (OPG )表达与PDGF BB浓度的关系 ;观察共育体系培养上清对OC吸附骨质的影响。结果 在PDGF BB刺激下 ,NO含量升高至 (2 5 .2 6±3 .2 4) μg/L (P <0 .0 1) ;加入抑制剂后 ,NO含量为 (9.84± 1.5 1) μg/L ;PDGF BB促进成骨细胞(OB)对eNOS和iNOS的表达 ,抑制剂使二者表达减弱 ;共育体系中OPG表达增强 ,而PDGF BB不能直接促进OPG的表达。共育体系培养上清使OC从骨质上脱离。结论 PDGF BB促进OB产生NO产物 ,后者抑制OC功能。
Objective To study the mechanism by which platelet-derived growth factor (PDGF)-BB inhibited osteoclastic bone resorption in osteoblast-osteoclast co-culture system.Methods PDGF-BB was administrated to osteoblasts and osteoblast-osteoclast co-culture system.The nitric oxide (NO) products in medium were measured,and the expression of nitric oxide synthases (NOS),including inducible NOS (iNOS) and endothelia NOS (eNOS),and osteoprotegerin (OPG) was detected by RT-PCR.The Vitronectin receptors on the membrane of osteoclasts were earmarked with immunofluorescence to appraise how the medium of co-culture system influenced the osteoclasts to adhere to bone slice.Results PDGF-BB stimulation could increase NO products from osteoblasts to (25.26±3.24) μg/L ( P <0.01),and NO was decreased to (9.84±1.51) μg/L after addition of the inhibitor.PDGF-BB promoted the expression of both iNOS and eNOS by by osteoblasts,while L-NAME could inhibit their production.The expression of OPG was increased in co-culture system,but PDGF-BB could not increase the OPG expression directly.The supernatant of the co-culture system could remove the osteoclasts from the bone slice.Conclusion PDGF-BB promoted osteoblasts to produce NO products,and NO inhibited osteoclastic bone resorption.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2004年第1期59-61,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目 (30 2 71 32 1 )
