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水稻Bowman-Birk蛋白酶抑制剂基因OsWIP1-2的诱导表达及其抑制活性 被引量：3

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摘要 克隆了水稻的WIP1基因,该基因属于Bowman-Birk蛋白酶抑制剂(BBI)家族.RNA杂交实验结果显示该基因的表达受伤害和茉莉酮酸的诱导,表明它在植物防御反应中起着重要的作用.将此基因克隆到表达载体pET28a,并在大肠杆菌中表达融合蛋白.分别用胰凝乳蛋白酶和胰蛋白酶为底物检测纯化的融合蛋白的抑制活性,发现融合蛋白具有胰凝乳蛋白酶的抑制活性,但没有对胰蛋白酶的抑制活性;同时发现融合蛋白C端的融合残基对蛋白酶抑制活性有重大影响,具有C端(His)6纯化标签的融合蛋白不具有胰凝乳蛋白酶的抑制活性,而有天然C端序列的融合蛋白具有胰凝乳蛋白酶的抑制活性,这暗示了过长的C末端序列可能会影响WIP1蛋白的正确折叠.蛋白酶抑制活性分析表明水稻Bowman-Birk抑制剂家族的成员可能在结构和功能上均发生了分化.
作者 陈军 刘静 郭蕾 瞿礼嘉 陈章良 顾红雅
机构地区 北京大学蛋白质工程及植物基因工程国家重点实验室
出处 《科学通报》 EI CAS CSCD 北大核心 2004年第7期657-661,共5页 Chinese Science Bulletin
关键词 水稻 Bowman-Birk蛋白酶抑制剂 WIP1 伤害诱导 茉莉酮酸
分类号 S511 [农业科学—作物学] Q78 [生物学—分子生物学]
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参考文献27

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二级参考文献12

共引文献13

同被引文献48

  • 1Rui-FengQI,Zhan-WuSONG,Cheng-WuCHI.Structural Features and Molecular Evolution of Bowman-Birk Protease Inhibitors and Their Potential Application[J].Acta Biochimica et Biophysica Sinica,2005,37(5):283-292. 被引量：17
  • 2江彤,杨建卿,高明,孔俊.不同抗病性烟草罹黑胫病后几种酶的活性及丙二醛含量的变化[J].安徽农业大学学报,2006,33(2):218-221. 被引量：25
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科学通报
2004年 第7期

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