摘要
以人淋巴细胞为抗原免疫BALB/c小鼠,然后取脾细胞与骨髓瘤细胞Sp2/0-Ag14融合,经以人淋巴细胞为抗原的间接ELISA初筛和以纯化的8_2m为抗原的间接ELISA筛选,获一株分泌抗β_2 m抗体的淋巴细胞杂交瘤。两次克隆化后,阳性率达100％。细胞表面抗原免疫荧光试验和兔抗人β_2 m抗血清阻断荧光抗体试验鉴定结果表明,此杂交瘤分泌β_2 m专一性抗体。该抗体为1gG_1。该杂交瘤命名为GA9。
HLA class I antigen light chain-β2-microglobulin((β2m) plays an important role in immunology and immunogenetics. In order to help the functional study and clinical use of β2m, the authours developed a hybridoma cell line. In this study, BALB/c mice were inoculated with human peripheral blood lymphocytes. After fusing it's spleen cell with Sp2/0-Agl4 myeloma cells (spleen cells: Sp2/0 cells 4:1), Authors set up 28 anti-lymphocyte clones. One of the clones (GAg) secreted antihuman |32m monoclonal antibody (McAb). The GAg hybrid has bsen cultured in vitro for four months, and secrets antibody continuously. The antibody secreted by GA9 clone reacted specifically with human β2m. The chromosome number of the GA9 hybridoma cell is gs.4 ++++ 2.28. This monoclonal antibody against human β2m was found to belong to subclass 1 of murine IgG.
出处
《中山医科大学学报》
CSCD
1992年第4期32-35,共4页
Academic Journal of Sun Yat-sen University of Medical Sciences
关键词
微球蛋白
单克隆抗体
杂交瘤
β_2-microglobulin
monoclonal antibody
lymphocytic hybridoma
