摘要
介绍了用斑点杂交技术,以Y染色体特异的3.5kb DNA片段为探针检测人血痕、人的组织碎块、牙齿、头发、羊水等的性别。样本经制备相当于0.1ul新鲜血的血痕,0.05 mg的组织块,带毛囊的头发1根,1/20颗牙齿牙髓质和2ul的羊水经本法检查,均能确定其性别。组织块经用福尔马林固定1周后,同样可以判断其性别,血痕性别的盲测结果全部正确。同时,对9种常见动物的血痕亦作了检查,均未显示阳性反应。本法准确灵敏,操作简单,值得进一步推广。
Results of sex determination of human bloodstains, tissue-debris, teeth, hairs, and amn-iotic fluids, by dot hybridization technique with Y chromosome specific 3.5 kb DNA probe, were reported. 0.1μl of fresh blood, 0.05mg of tissus-debris, one plucked hair, one 20th of a tooth pulp and 2μ1 of amniotic fluid were enough for sex determination by this method. The bloodstains stored longer than 4 years and the tissue-debris fixed by 10% formalin for one week could still be determined correctly. The blind test results of 6 bloodstains all were correct. These results showed that this method has many advantages, such as accuracy,sensitire, simple and fast so it is worthy to be spread in some laboratories.
出处
《中山医科大学学报》
CSCD
1992年第2期75-77,共3页
Academic Journal of Sun Yat-sen University of Medical Sciences
关键词
Y染色体
探针
斑点杂交
性别鉴定
Y chromosome specific probe
dot hybridization technique
sex identification
bloodstains
amniotic fluid
