摘要
苏云金芽孢杆菌 (Bacillusthuringiensis)是研究较深入、使用较广泛的杀虫细菌 ,野外使用易受阳光中紫外线的影响而失活。黑色素具有很强的抗辐射作用。将构建的含有嗜麦芽假单胞菌mel基因的质粒pWSY通过原生质体转化导入苏云金芽孢杆菌体内 ,使后者获得了稳定产生黑色素的能力。并在转化子细胞内检测到与供体菌相同的质粒。SDS PAGE显示该转化子体内额外表达了一分子量约为 1 8kD的蛋白 ,该蛋白很可能就是转化子表达的酪氨酸酶。经测定 ,转化子抗辐射作用明显增强 。
Bacillus thuringiensis was studied deeply and used wildly. It easily die from effect of ultra-violet ray when exposed in the sun. Antiradiation effect of melanin is very strong. Mel gene of P.maltophilia AT18 has been introduced into B.thuringiensis and enables it the ability of producing melanin steadily. The plasmid as the donor's has been tested in the transforment. SDS-PAGE analysis also revealed that an additional protein of 18kD, which was the tyrosinase expressed by the transforment. The results of assay show that antiradiation effect of trasformant is very strong, and the effect time of killing insect is longer than the recipient,while the fransforment cant infect animals and fowls.
出处
《微生物学通报》
CAS
CSCD
北大核心
2004年第2期72-75,共4页
Microbiology China
基金
江苏省社会发展基金资助项目 (No 135 2 0 0 10 16 )
关键词
嗜麦芽假单胞菌
苏云金芽孢杆菌
mel基因
转移
表达
Pseudomonas maltophilia, Bacillus thuringiensis, Tyrosinase geng, Transfer, Expression
