摘要
目的 探讨骨髓间充质干细胞心肌移植对大鼠心梗后心功能及钙泵的影响。方法 取大鼠骨髓间充质干细胞进行体外培养、增殖和标记 ,同时以液氮冷冻左室游离壁建立心梗模型。 4周后分别将 2× 10 6个细胞悬液或冷D -Hanks液注入实验组和对照组心梗区数个不同部位。另取 5只大鼠作为正常组 ,仅行两次开胸手术。 4周后行心功能测定及取标本 ,RT -PCR检测SERCA2基因mRNA的表达。结果 骨髓间充质干细胞于移植后 1周、2周和 4周后均可存活并具有分化成熟倾向。Buxco系统行有创动态心功能测定 ,发现SLVP、+dp/dt以正常组为最高 ,实验组次之 ,对照组最低。而LVEDP则相反以正常组最低 ,实验组次之 ,对照组为最高。心梗后SERCA2基因mRNA表达水平对照组较正常组明显降低 ( 0 988± 0 0 0 4对 1 82 4± 0 0 0 9,P <0 0 1)。骨髓间充质干细胞心肌移植后 ,虽然SERCA2基因mRNA表达水平实验组也较正常组有所降低 ( 1 5 83± 0 0 12对 1 82 4± 0 0 0 9,P <0 0 1) ,但与对照组相比明显升高 ( 1 5 83± 0 0 12对 0 988± 0 0 0 4,P <0 0 1)。结论 骨髓间充质干细胞心肌移植可促进SER CA2mRNA表达 ,并使大鼠心梗后受损的心功能改善。
Objective To investigate the effect of mesenchymal stem cells transplantation on heart function and the expression of Ca 2+ -ATPase (ERCA) mRNA after rat myocardial infarction. Methods Wistar inbred rat mesenchymal stem cells were cultivated, proliferated and labeled in vitro. Myocardial infarct models were set up by liquid nitrogen cryoinjuring the free wall of left ventricle. 4 weeks later, 2×10 6 mesenchymal stem cells or cold D-Hanks solution were injected into several different points of infarcted myocardial area of experiment group and control group rats. In addition, 5 Wistar inbred rats served as normal group only got thoracotomy twice. 4 weeks after transplantation, all rats received cardiac function test and specimens from infarcted area were got for the expression assay of SERCA2 mRNA by RT-PCR. Results Mesenchymal stem cells were alive at 1, 2 and 4 weeks after transplantation and had a trend toward differentiation and maturation in vivo. Traumatic dynamic measurement was applied to assay heart function by Buxco system, which showed that SLVP and +dp/dt were the highest in normal group, next in experiment group, and the lowest in control group, which LVEDP was the lowest in normal group, next in experiment group, and the highest in control group. The expression level of SERCA2 mRNA in control group was much lower than that in normal group(0.988±0.004 VS 1.824±0.009,P<0.01). Although SERCA2 mRNA expression in experimental group was lower than that in normal group (1.583±0.012 VS 1.824±0.009,P<0.01), it was obviously higher than that in control group(1.583±0.012 VS 0.988±0.004,P<0.01). Conclusion Mesenchymal stem cells transplantation can promote the expression of SERCA2, and improve damaged heart function after myocardial infarction.
出处
《中国医师杂志》
CAS
2004年第4期436-439,共4页
Journal of Chinese Physician
基金
中国博士后研究基金 (中博金 [2 0 0 0 ] 31号 )
湖南省社会发展计划项目 (0 3TCYZ0 1 2 )
美国中华医学基金项目 (CMB :99- 698)
