摘要
目的 研究活化蛋白 1(AP 1)在转化生长因子 β(TGF β)促进Ⅰ型胶原基因表达中的作用 ,探讨病理性瘢痕的形成机制。方法 用 5 μg/LTGF β刺激鼠成纤维细胞株NIH 3T3细胞 ,分别采用凝胶迁移变动分析 (EMSA)和逆转录 聚合酶链反应 (RT PCR)技术研究TGF β对AP 1的活化及其对α2 Ⅰ型胶原mRNA水平的影响。结果 5 μg/LTGF β刺激NIH3T3细胞 ,0 .5h即可检测到AP 1被活化 ,随时间推移AP 1的活性逐渐增强 ,6h达到峰值 ,而后活性逐渐减弱 ,2 4h仍然可检测到AP 1的活性。用TGF β刺激NIH3T3细胞 ,2 4h后提取总RNA ,经RT PCR分析 ,与对照组相比α2 Ⅰ型胶原mRNA水平明显增高。结论 TGF β刺激NIH3T3细胞 ,可激活AP 1并可以上调α2 Ⅰ型胶原的表达。
Objective To investigate the effect of AP 1 on TGF β promoting the expression of typeα2Ⅰcollagen mRNA.Methods NIH3T3 cells were treated with TGF β,and nuclear proteins were extracted,then AP 1 DNA binding activity were analyzed by EMSA; Total RNA was isolated and analyzed by RT PCR.Results AP 1 DNA binding activity could be observed after the cells were treated with TGF β for 0.5 h,and the pick point was at 6 h,and then AP 1 activity decreased greadually,but AP 1 activity could be detected at 24th h; After the cells were treated with TGF β for 24 h,the expression of α2Ⅰ collagen mRNA was increased significantly.Conclusion Not only could TGF β induce AP 1 DNA binding activity,but also it could promote the expression of type α2Ⅰcollagen mRNA relative to untreated control.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2003年第5期406-407,共2页
Chinese Journal of Experimental Surgery
基金
国家重点基础研究规划项目 (G1 9990 542 0 3)
国家自然科学基金资助项目 (C0 30 2 0 4 )
重庆市卫生局课题 (2 0 0 1 2 0 0 3)
