摘要
Objective: To investigate the urinary soy isoflavone metabolites from women, female piglets and rats fed with diet containing soy protein. Methods: Urinary samples from human and animals were collected after soy diet consumption. Identification for soy isoflavone metabolites in urine samples was processed using an Agilent Bruker LC Esquire ion trap system. Quantification of aglycone and conjugated soy isoflavone metabolites were also analyzed using a method published previously. Results: Identification studies showed that aglycones and conjugates of soy isoflavone metabolites were found in women and porcine samples. Interestingly, glucuronide conjugate of equol, besides glucuronide conjugates of genistein and daidzein, were found in rat urine. Glucuronide conjugate of equol was the major metabolite found in rat urine. A quantitative study showed that conjugated forms of isoflavones were more than 90% in woman urine, were between 80.5% and 84.5% in female porcine urine, and were less than 50% in female rat urine. Conclusion: Equol is the major metabolite found in female rat urine, but it is not found in woman or female porcine urine. Urinary profiles show that porcine model is more appropriate for mimicking human soy diet consuming studies.
目的 :研究妇人 ,母猪 ,雌性大白鼠食用后 ,尿中大豆异黄酮代谢物成份的比较。方法 :妇人食用大豆食品或以含大豆饲料喂食母猪 ,雌性大白鼠后 ,收集尿液 ,以液质联用分析仪分析尿液中大豆异黄酮代谢物的种类及含量。结果 :在尿液样品中检测出大豆异黄酮及其糖苷酸代谢物。在大白鼠尿液中的大豆异黄酮代谢物主要为 7 4’ 异黄烷二醇葡萄糖苷酸 (equolglucuronideconjugate) ,其次为 7,5 ,4’ 三羟基异黄酮糖苷酸 (genisteinglucuronideconjugate)及 7,4’ 二羟基异黄酮糖苷酸 (daidzeinglucuronideconjugate)。在妇人尿液中大豆异黄酮糖苷酸代谢物超过 90 % ,在母猪尿液中大豆异黄酮糖苷酸代谢物介于 80 .5 %～ 84 .5 %之间。在雌性大白鼠尿液中大豆异黄酮糖苷酸代谢物约有 5 0 %的大豆异黄酮糖苷酸代谢物。结论 :7 4’ 异黄烷二醇为大白鼠尿液中的主要大豆异黄酮代谢物。
基金
SupportedbygrantfromUSDepartmentofAgricultureAgricultureResearchServiceunderCRISProjectNumberUSDA ARS 62 5 1 5 10 0 0 0 0 3 0 6S 0 0 2
