摘要
目的 :分析腺苷受体的兴奋剂 2 -氯腺苷 (2 -CA)和非肽类磷酸抗原异戊烯焦磷酸 (IPP)在信号传导水平上对细胞内活性氧类 (ROS)的调节作用。方法 :SV4 0转化的人着色性干皮病成纤维细胞经 2 -CA和IPP处理 30min后 ,用流式细胞仪和荧光计测定细胞内ROS水平。结果 :在适当的控制条件下 ,IPP可降低细胞内 2 0 % - 30 %左右的ROS ,2 -CA对细胞内ROS调节类似IPP。结论 :2 -CA和IPP具有抑制细胞内ROS形成的作用 。
AIM: To study the effect of selective A1 adenosine receptor agonist, 2-chloroadenosine (2-CA), and non-peptide phosphoantigen isopentenyl pyrophosphate (IPP) on the intracellular reactive oxygen species (ROS) at the level of signaling event. METHODS: M1 cell line, derived from a subclone of the SV40-transformed xeroderma pigmentosum (XP) cell line, was treated with 2-CA, 8-br-cAMP, IPP at 37 ℃ incubator for 30 min, and intracellular ROS was measured by flow cytometry and CytoFluorometer. RESULTS: Under an appropriate control, 2-CA and IPP reduced intracellular ROS by 20%-30%. CONCLUSION: The data revealed that 2-CA and IPP inhibited cellular oxidant formation at the level of signaling event, suggesting both may have some anti-oxidant properties in vivo.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2003年第12期1627-1630,共4页
Chinese Journal of Pathophysiology
基金
东南大学新进博士科研启动基金资助项目(No .92 2 30 0 2 10 1)
关键词
2-氯腺苷
异戊烯焦磷酸
活性氧
抗氧化剂
2-chloroadenosine
Isopentenyl pyrophosphate
Reactive oxygen species
Antioxidants
