摘要
目的 探讨肿瘤转移抑制基因KAI1在喉鳞状细胞癌 (简称鳞癌 )中的表达及其与之发生、发展的关系。方法 采用原位杂交方法检测 84例原发性喉鳞癌 (primarylaryngealsquamouscellcarcinoma ,PLSCC)、2 7例喉癌前病变不典型增生 (laryngealprecancerouslesion ,LPL)、10例声带息肉(vocalcordpolyp ,VCP)和 10例正常喉黏膜 (normallaryngealtissues ,NLT)石蜡标本组织细胞中KAI1mRNA的表达。结果 NLT、VCP、LPL和PLSCC 4种组织中KAI1阳性表达的积分吸光度值 ( x±s)分别为 (136 2 0 6 8± 36 6 75 5 )、(1336 74 5± 4 2 85 8 5 )、(90 36 8 8± 2 5 70 1 9)和 (6 7880 6± 2 8189 5 ) ,其中NLT组和VCP组之间差异无显著性 (t=0 14 2 ,P >0 0 5 ) ,NLT组和LPL组之间差异有显著性 (t =4 2 81,P <0 0 1) ;PLSCC组中KAI1表达普遍下调 ,且病理分化G1 2组阳性表达水平高于G3组 ;T1 2病变组高于T3 4组 ;颈淋巴结NO组高于N1及N1以上组 ;临床Ⅰ Ⅱ期组高于临床Ⅲ Ⅳ期组 (P值均 <0 0 1)。KAI1表达与患者性别无关 (P >0 0 5 )。结论 KAI1低表达在喉鳞癌的发生、发展中可能起着重要作用 ,可望作为喉鳞癌早期诊断、评估肿瘤细胞侵袭转移潜能及患者病程发展阶段的指标之一。
Objective To investigate the expression of the metastasis suppressor gene KAI1 in laryngeal squamous cell carcinoma and the relationship between expression of KAI1 and it′s role in tumorgenesis and development. Methods The sections of 84 samples of primary laryngeal squamous cell carcinoma (PLSCC), 27 samples of laryngeal precancerous lesion (LPL),10 samples of vocal cords polyp(VCP) and 10 samples of normal laryngeal tissues (NLT) were examined by in situ hybridization for KAI1mRNA. Results It was found that 10 samples of NLT and 10 samples of VCP showed strong expression of KAI1, the Integrated A Total of KIA1 in these two types of tissues were 136 206.8± 36 675.536 and 133 674.505± 42 858.456, respectively, with no statistically significant difference between them,and high prevalence of decreased expression of KAI1 in LPL and PLSCC compared with that in VCP and in NLT ( P <0.01). There was a significant relationship between down-regulation of KAI1 expression with pathological grade, lymph node metastases, T stage and clinical stage ( P <0.01) and did not correlate with gender. Conclusion KAI1 may play an important role on tumor genesis, development, invasion and metastasis of PLSCC, and become one of the molecular markers for early diagnosis and predicting of aggressive and metastasis potential to assess the clinical stage of PLSCC.
出处
《中华耳鼻咽喉科杂志》
CSCD
北大核心
2003年第6期413-416,I002,共5页
Chinese Journal of Otorhinolaryngology
关键词
肿瘤转移
抑制基因
KAI1
喉鳞状细胞癌
基因表达
Genes, suppressor,tumor
Laryngeal neoplasms
Carcinoma,squamous cell
In situ hybridization
