摘要
目的 探讨E1A基因对人舌鳞状细胞癌淋巴结转移癌细胞裸鼠移植瘤生长的抑制作用和化学治疗 (化疗 )增敏作用及其相关机理。方法 通过裸鼠致瘤实验 ,观察了E1A基因的抑瘤作用。通过模拟E1A基因治疗临床应用的裸鼠实验 ,观察E1A基因及其与博来霉素联合应用在动物体内的疗效。采用免疫组织化学染色检测E1A基因对HER 2 /neu表达的影响。结果 裸鼠致瘤实验结果显示 :6 86LN 1 E1A细胞形成的肿瘤较 6 86LN 1和 6 86LN 1 vect的出瘤时间晚 ,生长慢 ,瘤重小 ,抑瘤率为 71 8%。模拟E1A基因治疗临床应用的裸鼠实验结果显示 :与对照组相比 ,博来霉素组、E1A基因组和E1A基因加博来霉素组的抑瘤率分别为 :5 3 1%、76 8%和 96 7% ,与博来霉素组相比 ,E1A基因加博来霉素组的抑瘤率为 :92 8%。免疫组织化学染色显示E1A基因能抑制HER 2 /neu的表达。结论 E1A基因能够明显抑制人舌鳞状细胞癌淋巴结转移癌细胞裸鼠移植瘤的生长并显著增加其对博来霉素敏感性 ,具有较好的临床应用前景 ,该作用可能与E1A基因抑制HER 2 /neu的表达有关。
Objective To study the effects of E1A gene on the growth and chemosensitivity of transplantation tumor of nude mice of lymph node metastasis cell line(686LN-1) of human tongue squamous cell carcinoma. Methods 686LN-1、686LN-1-vect and 686LN-1-E1A cells were transplanted into nude mice,then the time of tumor formation, growth rates and weight of tumor were observed. To study the effects of the E1A gene on bleomycin sensitivity in vivo, 686LN-1 cells were injected into nude mice. After tumor formed, bleomycin, E1A gene and E1A gene + bleomycin were given respectively as therapy. The tumor volume was calculated, and growth curve was plotted. Representative histologica1 sections were taken from mice bearing transplantation tumor either treated or control groups, and expression of HER-2/neu was detected. Results In nude mice, the expression of E1A gene significantly suppressed the growth rates and elongated the time of tumor formation. Bleomycin or E1A Gene and E1A gene + bleomycin can suppress the growth rates of transplantation tumor of nude mice, the suppressed rates of tumor growth was 53.13%﹑76.83% and 96.65%, respectively. The expression of E1A gene can significantly suppressed the expression of HER-2/neu gene in 686LN-1-E1A cells. Conclusion E1A is able to significantly inhibit the growth rate of transplantation tumor of nude mice of lymph node metastasis cell line(686LN-1) of human tongue squamous cell carcinoma,and dramatically enhance the sensitivity of the cells to cytotoxic agents in vivo. Above of all functions of E1A gene may be related to that it suppressed the expression of HER-2/neu gene.
出处
《中华耳鼻咽喉科杂志》
CSCD
北大核心
2003年第6期409-412,I001,共5页
Chinese Journal of Otorhinolaryngology
基金
国家自然科学基金资助项目 (3 0 0 70 85 6和 3 0 1710 5 8)
关键词
E1A基因
裸鼠移植瘤
生长抑制
化学治疗
增敏作用
人舌鳞状细胞癌
淋巴结转移
Adenovirus E1A proteins
Tumor cells,cultured
Receptor,erbB-2(HER-2 Proto-oncogene protein)
Tongue neoplasms
Lymphatic metastasis
Carcinoma,squamous cell
Drug therapy
Liposomes
