摘要
目的:了解肝硬化的基因表达概况,寻找在肝硬化及正常肝组织中差异表达基因.方法:以各24例肝硬化及正常肝组织的总RNA混合定量后反转录合成含有α-32pdATP的cDNA为探针,与Atlas微阵列表达分析膜杂交.结果:放射自显影结果经AtlasImageTM软件分析显示:在所分析的588种已知基因中,Integrinbeta7、collagentypeⅩⅧ等17个基因在肝硬化组织中表达上调,TFDP2、BAK、ABL等98个表达下调,参与细胞增生、凋亡、分化、细胞间相互作用、与细胞侵袭相关的基因表达水平发生了明显改变.结论:通过Atlas微阵列系统分析发现的这些基因的表达改变组成了—个肝硬化特异的基因表达谱.系统地研究肝硬化的基因表达改变,与肝硬化发生相关基因的差异变化可为肝硬化诊断和治疗提供线索.
AIM: To describe liver specific gene expression profiles and to identify genes with differential expression between liver cirrhotic tissues and normal liver tissues. METHODS: The cDNA probes which were labeled with α-32P dATP were synthesized from total RNAs of liver cirrhosis and normal liver tissues and hybridized to two identical Atlas human cDNA expression arrays membranes containing 588 known genes respectively. RESULTS: Autoradiographic results were analyzed by specific AtlaslmageTM (version1.01a) software. Among the 588 genes analyzed, 17 genes were found up-regulated in cirrhosis, including integrin beta 7 and collagen type ⅩⅧ, and 98 genes were down-regulated in cirrhosis, including TFDP2, BAK and ABL. Expression of the genes was associated with the regulation of cell proliferation, apoptosis, differentiation, cell-cell interaction, invasion regulators and cytokines altered. CONCLUSION: The results obtained from Atlas microarray provide a comprehensive liver cirrhosis specific expression profile. These results may be helpful for identification of target genes for diagnosis and designing rational therapeutic strategies.
出处
《世界华人消化杂志》
CAS
2004年第2期339-343,共5页
World Chinese Journal of Digestology
基金
黑龙江省自然科学基金资助项目
No.QC01C11~~
