摘要
目的:应用基因表达谱芯片研究丙型肝炎病毒非结构蛋白5A反式激活蛋白7(humangene7transactivatedbynonstructuralprotein5AofhepatitisCvirus,HCVNS5ATP7)的反式调节基因.方法:构建NS5ATP7基因的真核表达载体pcDNA3.1(-)-NS5ATP7,应用基因表达谱芯片技术对pcDNA3.1(-)-NS5ATP7转染的HepG2(人肝母细胞瘤细胞系)细胞和转染空载体pcDNA3.1(-)的相同细胞的差异表达mRNA进行检测和分析.结果:HepG2细胞经转染NS5ATP7后,有12条差异基因表达,其中4条基因表达增强,8条基因表达降低.结论:应用基因表达谱芯片成功筛选了NS5ATP7的反式调节基因,为进一步阐明NS5ATP7的反式激活作用及免疫调节机制提供了新的依据.
AIM: To study of genes trans-regulated by human gene 7 transactivated by nonstructural protein 5A of hepatitis C virus (NS5ATP7) by cDNA microarray assay. METHODS: The recombinant expression plasmid pcDNA 3.1(-)-NS5ATP7 was constructed, and HepG2 cells were transfected. Total mRNA was isolated from the HepG2 cells transfected with pcDNA3.1(-) and pcDNA3-NS5ATP7, respectively. Microarray was conducted for screening of up- and down-regulated genes of both HepG2 cells. RESULTS: After transfecting HepG2 cells, we found 4 genes were up-regulated, and 8 genes down-regulated. CONCLUSION: cDNA microarray is successfully used to screen the genes trans-regulated by NS5ATP7, which brings some new clues for studying the trans-regulated and immune regulation mechanism of NS5ATP7.
出处
《世界华人消化杂志》
CAS
2004年第2期319-322,共4页
World Chinese Journal of Digestology
基金
国家自然科学基金攻关项目
No.C03011402
No.C30070689军队"九
五"科技攻关项目
No.98D063军队回国留学人员启动基金项目
No.98H038军队"十
五"科技攻关青年基金项目
No.01Q138军队"十
五"科技攻关面上项目
No.01MB135~~
