摘要
目的:丙型肝炎病毒(HCV)的核心蛋白是HCV基因组编码的一种主要的结构蛋白,研究表明这种病毒的蛋白具有复杂的生物学调节作用.方法:以HCV的核心蛋白作为诱饵(bait),利用酵母双杂交技术,对于肝细胞表达型酵母细胞cDNA文库进行筛选.对于在缺陷型培养基上生长的真阳性酵母集落,进行回交实验,并对于cDNA文库表达载体质粒中插入的基因片段进行序列分析和生物信息学分析.结果:其中一个克隆命名为HCBP12,后来证明为视黄醇脱氢酶11(retinoldehydrogenase11,RDH11),或称为雄性激素调节的短链脱氢酶/还原酶1(androgen-regulatedshort-chaindehydrogenase/reductase1,ARSDR1).结论:这是首次发现和证实HCV核心蛋白与视黄醇脱氢酶11之间存在着相互结合和相互作用,为充分阐明HCV核心蛋白在HCV感染发病机制中的作用,开辟了新的研究方向.
AIM: To screen the hepatocyte protein interacting with HCV core protein. METHODS: By using HCV core protein as a bait, yeast-two hybrid system 3 was employed for screening and identification of HCV core protein-binding proteins from a hepatocyte cDNA library expressed in yeast cells. The protein-protein interaction was confirmed by back-cross experiment, and the inserts of the expressive vectors were sequenced and analyzed by bioinformatics methods. RESULTS: Among them we identified retinol dehydrogenase 11(RDH11/androgen- regulated short-chain dehydroge-nase/reductase 1 (ARSDR1) as a HCV core protein-interacting protein. This is the first time, to the best of our knowledge, to know the fact that there are interactions of HCV core protein-retinol dehydrogenase 11. CONCLUSION: The identification of retinol dehydrogenase 11 as the HCV core protein binding partner paves a new way for further understanding of the pathogenesis of HCV infection.
出处
《世界华人消化杂志》
CAS
2004年第2期286-290,共5页
World Chinese Journal of Digestology
基金
国家自然科学基金攻关项目
No.C03011402
No.C30070689
No.C39970674
No.C30070689军队"九
五"科技攻关项目
No.98D063军队回国留学人员启动基金项目
No.98H038军队"十
五"科技攻关青年基金项目
No.01Q138军队"十
五"科技攻关面上项目.No.01MB135~~
