摘要
目的 :观察趋化因子受体拮抗剂Met RANTES对同种异体大鼠异位小肠移植术后移植物的存活时间和组织病理学改变的影响 ,以及与小剂量他克莫司 (FK5 0 6 )的协同效应 .方法 :选用成年雄性SD和Wistar大鼠进行异位小肠移植 (SD→Wistar) .将动物分为 3组 ,每组 30只 :第 1组 ,未治疗对照组 ;第 2组 ,Met RANTES治疗组 (2 0 0 μg/d ,ip) ;第 3组 ,Met RANTES(2 0 0 μg/d ,ip) +小剂量FK5 0 6治疗组 [0 .5mg/(kg·d) ,im].观察移植大鼠的一般状况和存活时间 ,并于术后第 1 ,3,5 ,7日取各组动物移植肠标本进行组织病理学检查和组间比较 .结果 :第 1组大鼠存活时间中位数为 7.2d(1 .5 ) ,全部死于急性排斥反应及感染 ;组织病理学检查显示移植后第 3,5 ,7日分别符合轻、中、重度排斥反应 .第 2组大鼠存活时间中位数为 1 9.2d (1 6 .4 ) ,与第 1组相比存活时间明显延长 (P <0 .0 1 ) .第 3组大鼠存活时间中位数为 30 .9d(9.0 ) ,与第 1 ,2组显著延长 (P <0 .0 1 ) .第 2 ,3组组织病理学检查无明显排斥反应征象 ,可长期存活 .结论 :Met RANTES能明显抑制小肠移植急性排斥反应 ,有效保护移植肠功能 ,显著延长移植物的存活时间 ,并可增强小剂量FK5 0 6的免疫抑制作用 .
AIM: To observe the effects of chemokine receptor antagonist, Met RANTES, on the survival duration and pathological changes of allograft rats of heterotopic small bowel transplant, and the coordinative effects of Met RANTES used together with low dose of FK506. METHODS: Heterotopic small bowel transplant was performed between mature male SD and Wistar rats (SD rat small bowels transplanted into Wistar recipients). According to the different treatment, rats were divided into 3 groups: Group 1, allogeneic small bowel transplant untreated group as control, Group 2, allogeneic small bowel transplant treated with Met RANTES(200 μg/d,ip), and Group 3, allogeneic small bowel transplant treated with Met RANTES(200 μg/d,ip)and low dose of FK506 [0.5 mg/(kg·d), im]. Post transplant clinical course and survival duration were recorded and the grafts were sampled at day 1, 3, 5, and 7, respectively, after the transplant. All samples were examined pathologically and compared across the groups. RESULTS: The survival duration median of the first group was 7.2 days (1.5) and the cause of death of all recipients was acute rejection and infection. The pathological examination of the allografts from the first group demonstrated mild, moderate, and severe rejections on post operative day 3, 5, and 7. The survival duration median of the second group was 19.2 days (16.4), obviously longer than that of the first group ( P <0.01). The survival duration median of the third group was 30.9 days (9.0), significantly longer than that of the other two groups ( P <0.01). The pathological examination of the allografts from the second and third groups demonstrated no obvious indication of rejection. CONCLUSION: Met RANTES may obviously suppress the allograft rejection, effectively protect the function of allograft, and significantly prolong the survival duration of the recipients. In addition, Met RANTES can strengthen the immunosuppression effects of low dose of FK506.
出处
《第四军医大学学报》
北大核心
2004年第5期449-451,共3页
Journal of the Fourth Military Medical University
关键词
趋化因子
小肠移植
移植物排斥
免疫抑制
chemokine
small bowel transplantation
graft rejection
immunosuppression
