摘要
目的 :讨论抗凋亡基因bag 1在乳腺癌组织中的表达及意义 ,了解肿瘤细胞凋亡的特点 .方法 :采用免疫组化SABC法观察了 1 0 0例乳腺癌与 1 0例乳腺良性肿瘤及 1 0例正常乳腺组织的石蜡标本切片中bag 1的表达 .结果 :bag 1在乳腺癌组织、乳腺良性肿瘤和正常乳腺组织中阳性表达率分别为 85 .0 % ,1 0 .0 %和 1 0 .0 % ,差异明显 (P <0 .0 5 ) .在I期 ,II期和III期的乳腺癌组织中bag 1阳性表达率分别为87.5 % ,82 .4 %和 88.2 % .三者无明显差异 (P >0 .0 5 ) .在导管癌、小叶癌及特殊型癌中bag 1阳性表达率分别为 85 .5 % ,84 .8%和 80 .0 % .无明显差异 (P >0 .0 5 ) .对 1 0 0例乳腺癌进行随访 ,bag 1阳性表达者 5a生存率为 78.7% ,bag 1阴性表达者 5a生存率为 8.5 % ,两者差异明显 (P <0 .0 5 ) .结论 :bag 1在乳腺癌组织中高表达 ,bag 1在乳腺癌组织中的表达与肿瘤的临床分期、病理分型等无关 .bag 1可以作为早期诊断乳腺癌的一种生物标记 .bag 1可以作为乳腺癌预后的一个生物指标 .
AIM: To evaluate bag 1 expression in breast cancer and to evaluate its clinical significance. METHODS: One hundred samples of breast cancer specimens were analyzed by SABC immunohistochemical stain for anti apoptotic gene bag 1 expression. RESULTS: The positivity rate of bag 1 expression in breast cancer, benign tumor and normal tissues was 85.0%, 10.0%, 10.0% respectively,and significant difference was observed ( P <0.05). The bag 1 expression rate in pathologic grade I, II, III was 87.5% , 82.4%, 88.2% respectively, and no significant difference was observed ( P >0.05). The positivity rate of bag 1 expression in duct carcinoma, lobular carcinoma and special carcinoma was 85.5%, 84.8%, 80.0% respectively, and no significant difference was observed ( P > 0.05). Following up 100 breast cancer patients showed that 5 year survival rate of bag 1 positive patients was 78.7%, and that of the bag 1 negative ones was 8.5%, significant difference was observed ( P <0.05). CONCLUSION: There is an overexpression of bag 1 in breast cancer. The positivity rate of bag 1 expression is not related to the pathologic grade and the clinical type. bag 1 might serve as a biomarker to diagnose and prognose breast cancer in the earlier period.
出处
《第四军医大学学报》
北大核心
2004年第3期282-284,共3页
Journal of the Fourth Military Medical University
