摘要
目的 :探讨巢蛋白 (nestin)在胚胎后期、新生期、成年三阶段的核酸、蛋白水平表达的变化 .方法 :取三个发育时期的胰腺 ,应用RT PCR、免疫组化的方法进行半定量、定位分析 .结果 :在胚胎后期、新生期、成年三阶段nestin在核酸、蛋白水平均有表达 ,阳性细胞主要分布在胰岛 ,在腺泡周围的外分泌中有少量分布 .结论 :随大鼠胰腺的生长发育 ,核酸、蛋白表达下调 .
AIM: To study the expression of nestin in the levels of protein and nucleotide in pancreas of E18.5, newborn and adult rats. METHODS: The mRNA of nestin was amplified by RT PCR, and the number of nestin positive cells and the levels of nestin protein were investigated by immunohistochemistry. RESULTS: Nestin was expressed in E18.5, newborn and adult phases. Nestin positive cells were mostly located in islets and slightly in periacinal exocrines. CONCLUSION: With the development of rat pancreas,the expression of nestin in the levels of nucleotide and protein declines, the percentage of nestin positive cells in islets also decreases.
出处
《第四军医大学学报》
北大核心
2004年第3期193-196,共4页
Journal of the Fourth Military Medical University
基金
江苏省自然科学基金 (BK2 0 0 1 1 1 9)
