摘要
提出了一种基于液芯波导 ( Liquid core waveguide,LCW)原理的微流控芯片吸收光度检测系统 .通过芯片与外界接口技术实现液芯波导管与芯片的耦合 ,建立了芯片上长光程 (毫米至厘米级 )吸收光度检测池 .采用邻菲啉 -铁 ( )显色体系验证系统分析性能 ,以 5 .5 cm外覆 Teflon AF液芯波导管作为检测池 (检测池体积 2 40 n L)时 ,芯片系统的检测线性范围为 0 .0 3～ 5 0μmol/L,对邻菲啉 -铁 ( )配合物的检出限为8nmol/L,检测池有效光程达 1 .7cm,分析精度 RSD( n=5 )为 0 .8% .
In this work, a system for high sensitivity absorbance measurements on microfluidic chips was developed with a detection volume of 240 nL. Long optical path-length(1.7 cm effective path length) was achieved on the chip by using an integrated liquid core waveguide(LCW) silica capillary coated with Teflon AF. The performance of the system was tested by using o-phenanthroline-Fe(Ⅱ) complex as a model sample with spectrophotometric detection, achieving a detection limit of 8 nmol/L for the complex. The absorbance of samples ranging from 0.03—50 μmol/L is linear(R 2=0.999 4) and showed good repeatability with a RSD of 0.8%(n=5). The sensitivity of the chip-LCW system was a factor of 1.7 higher than conventional spectrophotometry using a 1 cm cell. The present system can be applied in continous flow or flow injection analysis systems.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2004年第4期610-613,共4页
Chemical Journal of Chinese Universities
基金
国家自然科学基金重大项目 (批准号 :2 0 2 990 3 0 )
教育部科学技术研究重点项目 (批准号 :0 10 93 )资助
