摘要
目的 :研究山莨菪碱对大鼠肝星状细胞增殖的影响 ,为应用山莨菪碱防治肝纤维化提供实验依据。方法 :采用链霉蛋白酶、胶原酶原位灌流 ,以 Nycodenz为分离介质 ,单层一步密度梯度离心法 ,分离大鼠的肝星状细胞。并以 MTT比色法观察山莨菪碱对肝星状细胞的增殖效应 ,用流式细胞仪检测了肝星状细胞在细胞周期中 DNA含量的改变。结果 :山莨菪碱能明显抑制细胞增殖 ,并呈药物浓度依赖关系 ,DNA合成受到抑制。结论 :山莨菪碱在体外具有抗肝纤维化的作用 。
Objective: To study the effect of Ani on hepatic satellate cell (HSC) proliferation of Wistar rats and offer experimental basis for preventing and treating hepatic fibrosis with Anisodamine(Ani). Methods: HSLs were isolated by perfusion with pronase and collagenase and density gradient centrifugation with Nycodenz; MTT colorimetric assay was used to observe the effect of Ani on HSC proliferation; DNA changes in cell cycle with flow cytometer(FCM).Results:Ani dramatically inhibited HSC dose-dependent proliferation and the synthesis of DNA. Conclusion: Ani shows anti-hepatibrosis effect in vitro and may be significant and applicable in liver fibrosis.
出处
《黑龙江医药科学》
2004年第2期32-33,共2页
Heilongjiang Medicine and Pharmacy
关键词
肝星状细胞
肝纤维化
山莨菪碱
增殖
hepatic satellate cell
hepatic fibrosis
Anisodamine
proliferation
