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锯缘青蟹不同器官组织中ROS与ASAFR含量的研究 被引量:2

Comparative study on content of reactive oxygen species and activity of antisuperoxide anion free radical in different organs and tissues of mud crab, Scylla serrata
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摘要 采用生化测定的方法,对锯缘青蟹(Scyllaserrata)鳃、肝胰腺和肌肉等不同器官组织中活性氧(ROS)与抗超氧阴离子自由基(ASAFR)含量进行了测定。 结果表明, 不同器官组织中活性氧与抗超氧阴离子自由基含量各异。 各器官组织中活性氧含量由高到低依次为:肝胰腺>肌肉>鳃, 分别为195.04U/mg±33.46U/mg, 64.03 U/mg±12.77 U/mg和44.84U/mg±10.44U/mg。而抗超氧阴离子自由基含量则是鳃>肝胰腺>肌肉,大小分别为439.62 U/g±64.73U/g,297.89 U/g±39.75 U/g和84.24 U/g±18.03 U/g。统计分析显示,锯缘青蟹各器官组织间的活性氧与抗超氧阴离子自由基含量均存在极显著性差异(P<0.01)。这是由于不同器官组织所执行的生理功能不同造成的。 This study determined content of reactive oxygen species (ROS) and antisuperoxide anion free radical (ASAFR) in the gill, hepatopancreas and muscle of mud crab, Scylla serrata using biochemical methods. Results showed that content of ROS and ASAFR vary between different organs and tissues. ROS content is hepatopancreas>muscle>gill, with values of 195.04 U/mg±33.46 U/mg, 64.03 U/mg±2.77 U/mg and 44.84 U/mg ±0.44 U/mg,respectively. ASAFR content is gill>hepatopancreas>muscle, the values are 439.62 U/g±4.73 U/g, 297.89 U/g±9.75 U/g and 84.24 U/g±8.03 U/g, respectively. Statistical analysis indicates that content of ROS or ASAFR between the various organs and tissues varies significantly (P<0.01) due to their different physiological functions.
出处 《海洋科学》 CAS CSCD 北大核心 2004年第4期1-4,共4页 Marine Sciences
基金 国家863 重大专项(2002AA603013) 国家自然科学基金(39870563) 福建省重中之重"福建省海洋生物优良种质及生物活性物质应用基础研究"部分资助项目
关键词 锯缘青蟹 器官组织 ROS ASAFR 肝胰腺 肌肉 活性氧 抗超氧阴离子自由基 mud crab Scylla serrata gill hepatopancreas muscle reactive oxygenspecies(ROS) antisuperoxideanion free radical (ASAFR)
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