摘要
用mRNA差异显示法对7℃低温胁迫3 d后的香蕉幼苗叶片进行研究,回收了18条在低温下水杨酸(salicylic acid,SA)诱导的差异带;反向Northern杂交证明,SA在低温胁迫下能诱导7条差异带高表达。对其中最显著表达的两条差异带(G和A)进行克隆和测序,结果显示,G片段序列与大豆在冷胁迫环境下两个高表达基因片段的部分序列有92％同源性;A片段未发现其同源性 基因片段。
The mRNA differential display method was applied to identify banana genes that were regulated by salicylic acid (SA) during chilling stress. Eighteen cDNA fragments induced by SA during chilling stress were retracted. Seven of them were affirmed by reverse Northern hybridization to be significantly induced. Two most differential fragments (G and A) of them were cloned and sequenced. Nucleotide sequence analysis showed that clone G fragment had 92% homology to partial cDNA sequences of two cold-related genes in soybean (Glycine max) and clone A did not show any identity to previously reported sequences in GenBank database.
出处
《植物生理与分子生物学学报》
CAS
CSCD
2004年第2期225-228,共4页
Journal Of Plant Physiology and Molecular Biology
基金
Supported by a key research grant(No.1 999-11)from the Educational Committee of Guangzhou City,China
关键词
香蕉
抗寒性
MRNA差异显示
基因表达
水杨酸
banana
chilling tolerance
mRNA differential display
gene expression
salicylic acid
