摘要
35S-Met标记玉米胚蛋白合成结果表明,热激处理(42℃)与对照(25℃)的蛋白合成趋势相近,热激抑制16 DAP的蛋白合成,增加22和34 DAP蛋白合成。SDS-PAGE自显影图谱表明,热激诱导16DAP的胚合成86.4、80.0、73.2 kD等3种分子量较高的热激蛋白,22 DAP后热激诱导合成86.4、80.0、73.2、24.4、18.2、16.8和13.6 kD等7种分子量的热激蛋白。2D-PAGE自显影图谱进一步显示,热激诱导22和28 DAP的胚合成近20种热激蛋白,其中超过10种为小分子热激蛋白。特异热激蛋白BiP(HSP70)、PDI(HSP60)Western blot表明,这2种热激蛋白在玉米胚发育过程均有高水平的表达,热激对其合成影响不明显。
Labeling of protein in developing maize embryo with 35S-Met showed that, the protein synthesis rate in heat shock (42°C) was similar to that in the control (25°C). Heat shock inhibited protein synthesis at 16 DAP, whereas it stimulated protein synthesis at 22 and 34 DAP (Fig.2). The pattern of SDS-PAGE showed that (Fig.3), a new set of heat shock proteins were induced after heat shock. At 16 DAP, heat shock induced 3 kinds of HSPs with molecular weight 86.4, 80.0 and 73.2 kD. And at 22 DAP, 7 kinds HSPs were induced with molecular weight 86.4, 80.0, 73.2, 24.4, 18.2, 16.8 and 13.6 kD. After 22 DAP, except 3 kinds of protein with molecular weight 73.2, 24.4 and 18.2 kD were induced turnover synthesis, other 4 kinds were increased by heat shock. The pattern of 2D-PAGE further suggested that (Fig.4), heat shock induced more than 20 kinds of HSPs at 22 and 28 DAP, especially the smHSP of 20-30 kD were more than 10 kinds. Western blot indicated that(Figs.5,6), BiP (HSP70) and PDI (HSP60) kept a high expression level during the embryo development, and failed to respond to heat shock.
出处
《植物生理与分子生物学学报》
CAS
CSCD
2004年第2期161-166,共6页
Journal Of Plant Physiology and Molecular Biology
基金
国家自然科学基金项目(No.30370912)
中山大学青年教师科研启动基金资助~~
关键词
玉米
胚发育
热激蛋白
maize(Zea mays L.)
embryo development
heat shock protein
