摘要
为检测雌激素受体在绝经后妇女可能的作用 ,采用免疫荧光法检测卵巢切除小鼠脑内雌激素受体 (ER)的分布。结果清楚表明 ,ERα除了存在于已知的下丘脑腹内侧核、下丘脑前区中央部和弓状核外 ,还见于室旁核和视上核 ,而文献报道 ERβ在该区域占主导地位。我们还首次报道 ERα在正中隆起的分布。如期所见 ,ERα还分布于终纹床核和杏仁核。尽管试用多种来源的商品化 ERβ抗体 ,也未检测到脑内 ERβ免疫反应阳性细胞 ,推测可能是脑内 ERβ蛋白含量稀少的原因。在脑内广泛存在 ER,并且 ER在不同核团有独特分布 ,这就有可能针对雌激素广泛作用中某一特殊功能设计药物。
The distribution of estrogen receptors(ER) in the brain of ovariectomized mice was further examined by immunoflurescence method, in order to detect the possible role of ER in postmenopausal women. The results clearly demonstrated that apart from the existence of ERα in known parts of hypothalamus at high concentration, such as ventricular medial nucleus, central part of anterior hypothalamus and arcuate nucleus, ERα also existed in the paraventricular nucleus and supraoptic nucleus, arguing against the reported ERβ predominance in these areas. ERα was first detected in the medial eminence in our study. As expected, ERα existed widely in bed nucleus of the stria terminalis and amygdala, but were absent in the cortex and hippocampus in contrast to the reported ERβ mRNA distribution. The detection of ERβ immunoreactive cells in brain did not succeed even applying with several commercial sources of antibodies to ERβ. We could only reason that ERβ protein in mice brain might be scarce. The widely existence of ER and the distinct distribution in brain raise the possibility of pharmaceutical interventions targeting specific aspects of estrogen's wide range of functions.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2004年第2期175-178,共4页
Chinese Journal of Neuroanatomy
