摘要
目的 :探讨膀胱肿瘤患者外周血中CK 2 0mRNA表达的临床意义。方法 :采用RT PCR技术检测 32例膀胱癌患者手术前、10例手术后及 10例健康者外周血标本中CK 2 0mRNA的表达。结果 :在手术前外周血中CK 2 0mRNA表达阳性率为 15 .6 % (5 / 32 ) ;在手术后外周血中CK 2 0mRNA表达阳性率为 2 0 .0 % (2 / 10 ) ,其中 1例术前外周血CK 2 0mRNA表达阴性 ,手术后变为阳性 ;健康者外周血均无CK 2 0mRNA表达。膀胱癌患者外周血中CK 2 0mRNA表达阳性率与肿瘤大小、数目无显著关系 (P >0 .0 5 ) ,但随着肿瘤分级、分期的增加 ,CK 2 0的检出率似有增加的趋势。结论 :以RT PCR技术检测膀胱癌患者外周血中CK 2 0mRNA的表达 ,可早期诊断和监测膀胱肿瘤细胞血行微转移。
Objective:To explore the clinical significance of detecting the cytokeratin 20(CK-20) mRNA in peripheral venous blood of patients with bladder carcinoma.Method:Reverse transcriptase polymerase chain reaction(RT PCR) was performed to detect the expression of CK 20 mRNA in peripheral venous blood samples of 32 bladder cancer patients, 10 healthy volunteers and 10 postoperation cases with bladder cancer.Result:CK 20 mRNA amplification band was detected in 5 cases with bladder carcinoma(5/32, 15.6%), 2 postoperatiopn patients(2/10, 20.0%), not obtained in all 10 healthy volunteers. The postive rate of CK20 mRNA expression seemed to increase with the grading and staging of bladder cancer, but had no relation with the number and size of bladder neoplasm.Conclusion:Detection of peripheral blood CK20 mRNA expression by means of RTPCR might indicate the hematogenous micrometastasis of bladder cancer cells and help to diagnose bladder carcinoma in earlier period.
出处
《临床泌尿外科杂志》
2004年第4期221-223,共3页
Journal of Clinical Urology
基金
重庆市教委科学技术研究项目 (渝教科 [1999]16 )
