摘要
选用110只雄性Wistar大鼠,落体致伤法造成大鼠脑损伤模型,放免法检测伤后3小时内4个时限鼠脑挫伤皮质血栓素B_2(TXB_2)与6—酮—前列腺素F_(1a)(6—Keto—PGF_(1a))含量,并通过电镜、光镜检查和伊文思蓝染范围,探讨血栓素A_2(TXA_2)、前列环素(PGI_2)与继发性脑损害的关系以及川芎嗪的作用。结果:挫伤脑组织TXB_2、6—Keto—PGF_(1a)含量在伤后逐渐增高,以3小时增高较著。TXB_2/6—Keto—PGF_(1a)值(T/K值)也逐渐递增;病理检查证实,随伤后时间延长,脑损害程度愈加重。应用川芎嗪治疗后,脑组织TXB_2含量明显降低,T/K值缩小;结果提示:伤后TXA_2和PGI_2这一对微循环调节因子的代谢失衡是加重继发性脑损害的重要因素之一,川芎嗪可对抗TXA_2的生成与活性,减轻TXA_2与PGI_2的代谢失衡,使挫伤脑组织缺血缺氧状态得以部分改善。
110 male Wistar rats were used to make a model of head injury by a weight drop device, and the contents of TXB_2 and 6-Keto-PGF_(1a)in damaged cerebral tissue were measured at 4 time limit 3 hours after injury by way of RIA. Morever. observation of the spreading of Evans blue in cortical tissue with light and electron microscopy were undertaken to study the correlation between secondary brain injury and Thromboxane A_2 (TXA_2), and prostacyclin (PGI_2) levels as well as the effects of Ligustrazini Hydrcohlrioi. The results showed that the concentration of TXB_2 and 6-Koto-PGF_(1a) was progressively increased within 3 hours after injury, and the ratio between TXB_2 and 6-Keto-PGF_(1a) (T/K value) increased during the early postinjury period, meanwhile, the tissue damage was more aggrevated; after the use of Ligustrazini Hydrochirioi content of TXB_2 in the brain decreased sharply accompanied by drop in T/K value. It is suggested that the metabolic imbalance between TXA_2 and PGI_2 may be one of the important factors in the development of secondary brain injury. Ligustrazini Hydrochirioi can inhibit the synthesis and activity of TXA_2 and abate the inbalance between TXA_2 and PGI_2, as a result, brain ischemla and hypoxia are partially improved.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
1992年第2期85-88,共4页
Chinese Journal of Trauma
关键词
脑损伤
血栓素A2
前列环素
Head injurv Thromboxane A_2 Prostacyclin Lignstrazini hyddrochrioi
