摘要
应用胶体金免疫电镜技术对8例流行性出血热(EHF)电者骨髓细胞中病毒抗原进行了检测,结果S例骨髓细胞病毒抗原均阳性,主要定位于细胞膜,且变性的骨髓细胞胞浆内也有特异性金颗粒存在。同时骨髓细胞超微结构也可见线粒体变性,空泡病变区等改变。以上说明流行性出血热病毒(EHFV)能主动侵入骨髓细胞:并在其中繁殖复制与EHF的免疫功能异常及免疫发病机理有密切的关系。
Detectton of viral antigen in the marrow cells of epidemic hemorrhagic fever(EHF)patients was perfomed by protejn A-gold technique using electron microscope.Olesr specificgold partioleg were observed.All marrow cells showed some degree of ultrastructureabnormalities suoh as degenoration and vaouolization.The above results suggerted that themarrow cells served as a site of viral replication and accumulation,and thus resulted in.deficienoy of immune funotion in EHF patients.
作者
曹廷华
巫振欧
管美英
袁光辉
张传生
刘新梅
CAO TAN-HUA(The First Afiliated Hospital of Hubei Medical college,Hubei)
出处
《中华传染病杂志》
CAS
CSCD
北大核心
1992年第2期93-95,共3页
Chinese Journal of Infectious Diseases
关键词
胶体金
免疫电镜
流行性出血热
Epidamio hemorrhagic fever
Marrow cell
Protein A-gold technique
