摘要
目的 研究皮肤来源的前体细胞 (SKPs)的体外培养方法 ,为神经移植提供一种新的细胞来源。方法 分离培养小鼠皮肤组织的细胞 ,在无血清的培养基中培养 ,用机械方法对细胞进行传代 ,免疫细胞化学方法对细胞进行鉴定。结果 从成年和幼年的小鼠皮肤组织中分离培养出SKPs ,这种细胞在体外可以长期增殖和传代 ,体外培养可超过 8代 ,这种细胞大部分表达纤维粘连蛋白 ,约50 %的细胞表达巢蛋白。在有血清的培养基中培养 ,约 5%的细胞分化为神经元样细胞 ,表达神经元特异性烯醇化酶和神经微丝 ,部分细胞分化为脂肪细胞 ,其余的细胞分化为成纤维细胞样细胞。结论 皮肤组织中存在的前体细胞可在体外稳定增殖 ,能够分化为神经细胞、脂肪细胞和成纤维细胞样细胞。
ObjectiveTo investigate the culture conditions of skin-derived precursors (SKPs) and to explore a new cell source for central nervous system cell transplantation.MethodCells from skins of juvenile and adult mice were isolated and cultured in serum -free medium, and mechanical methods were adapted to passage these cells and ce lls were identified by immunocytochemistry.ResultsA population of SKPs could be isolated from adult and neonatal skins. They co uld be maintained in vitro for extended periods with stable population doubling, and they were expanded as undifferentiated cells in culture for more than 8 pas sages, indicating their proliferative capacity. About 50?% of SKPs expressed n estin and the majorities of these cells expressed fibronectin when they were pla ted on polyornithine and laminin coated plates. About 5?% cells showed typical complicated neuronal states and expressed NF-M and NSE when SKPs were plated i n serum-containing medium. These cell could also differentiate into adipocytes and fibroblast-like cells.ConclusionsAdult skin contains stem cells capable of differentiating into neurons, adipocyt es and fibroblast-like cells. SKPs may represent an alternative autologous stem cell source for CNS cell transplantation.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2004年第2期106-108,共3页
Chinese Journal of Organ Transplantation
基金
国家自然科学基金资助项目 (39993430)
