摘要
目的 探讨L 精氨酸对任意型皮瓣的成活的影响。方法 以Wistar大鼠为实验对象 ,在其背部设计 7cm×2cm任意型皮瓣 ,于术后给予腹腔注射L 精氨酸 30 0mg/kg ,对照组给予 0 9%生理盐水。术后 7d ,通过图像分析技术观察皮瓣成活率 ;通过生物化学技术、组织学和免疫组织化学技术对不同时间皮瓣组织中一氧化氮含量、组织形态学变化、白细胞计数以及ICAM 1的表达进行观测。结果 外源性L 精氨酸可提高皮瓣组织一氧化氮含量 ,L 精氨酸组ICAM 1表达呈弱阳性、术后 12h皮瓣组织真皮中性粒细胞计数减少 ,L 精氨酸组皮瓣成活率明显高于对照组 (P <0 0 1)。结论 外源性L 精氨酸可提高皮瓣组织NO含量 ,ICAM 1表达下调 ,中性粒细胞浸润减少 ,提高任意皮瓣成活率。
Objective To investigate the effect of L-arginine on the survival of random pattern skin flaps. Method The random pattern skin flaps on the back of rats were utilized to observe the survival rate of the skin flaps, the nitric oxide concentration, the neutrophil count, and the ICAM-1 expression by means of image analysis technology, biochemical and immunohistochemical methods.Result The survival rate of skin flaps in the L-arginine-treated group increased significantly ( P <0.01) when compared with the control group at day 7 after the operation. The concentrations of nitric oxide in the tissues of the L-arginine-treated group were different from the control group at 8, 12, 24, and 72 hours after the operation. The neutrophil count in the L-arginine-treated group was less ( P <0.01) than that in the control group. The immunohistochemical reaction of ICAM-1 was stronger in the control group.Conclusion L-arginine can elevate nitric oxide concentrations in random pattern skin flap tissues and protect skin flaps from neutrophil-mediated ischemia-reperfusion injury.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2003年第3期268-271,共4页
Chinese Journal of Histochemistry and Cytochemistry
基金
湖北省自然基金 (30 1130 5 6 6 )
