摘要
采用HPLC快速柱同时测定盐酸丁卡因及其分解物对丁氨基苯甲酸的含量。在0.08～0.24mg/ml(0.16～1.60μg/ml)范围内盐酸丁卡因(对丁氨基苯甲酸)浓度与峰面积成良好线性关系。盐酸丁卡因的方法回收率为100.23%,CV=0.27%;对丁氨基苯甲酸的方法回收率为99.97%,CV =1.11%。
Tetracaine hydrochloride and p-butylaminobenzoic acid were determined by HPLC fast-column.Experiments were performed on a Model YSB-2 liquid chromatograph equipped with a single-wavelengthUV detecter (Model K_(401) 251). The instrument was fitted with a 50×4.6mm I. D. YWG C_(18) reversed-phase fast-column. The mobile phase was methanol/aqueous/ethylenediamine (60/40/0.08) the pH of thesolution was adjusted to 6.72 using glacial acetic acid. The standard curve of tetracaine hydrochloride (p-butylaminobenzoic acid) was linear within the concentration range 0.08～0.24mg/ml (0.16～1.60μg/ml). The average recoveries of tetracaine hydrochloride and p-butylaminobenzoic acid were 100.23% (CV=0.27%) and 99.97% (CV=1.11%), respectively.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
1992年第8期346-348,共3页
Chinese Journal of Hospital Pharmacy
关键词
HPLC
快速柱
盐酸丁卡因
含量
测定
HPLC
fast-column
Tetracaine hydrochloride
P-butylaminobenzoic acid
