摘要
目的 探讨纳米材料作为组织工程骨基质材料的可行性。方法 分离培养兔骨髓间充质干细胞 ,诱导为成骨细胞后作为种子细胞 ,与纳米晶羟基磷灰石胶原材料于体外联合培养 ,通过对复合物光镜、免疫组织化学染色及扫描电镜观察 ,了解细胞在材料中生长情况。结果 兔骨髓间充质干细胞可以诱导为成骨细胞 ,体外复合培养 8天 ,分布于支架材料上的细胞大量分化增殖、分泌细胞外基质。结论 纳米晶羟基磷灰石胶原材料是一种构建组织工程骨的较好的支架材料。
Objective To investigate the feasibility of the nanometer crystal hydroxyapatite collagen material as scaffold material in bone tissue engineering.Methods Osteoblasts,which were induced from mesenchymal stem cells isolated from rabbits bone marrow,were cultured as the seeding cells,then the cells were cocultured with the nanometer crystal hydroxyapatite collagen material in vitro.The cell-material complex was observed under light microscope,immunohistochemistry staining and electronic scanning microscope in order to evaluate the interacting between cells and material. Results It is posible to induce mesenchymal stem cells,isolated from bone marrow,into osteoblasts.Eight days after coculture,the osteoblasts differentiated and proliferated in the nanometer crystal hydroxyapatite collagen material network.Extracellular matrix was secreted.Conclusion The nanometer crystal hydroxyapatite collagen material is a good scaffold material for the remodeling bone in bone tissue engineering.
出处
《江苏医药》
CAS
CSCD
北大核心
2003年第11期810-812,F002,共4页
Jiangsu Medical Journal
基金
无锡市社会发展项目资助 (CS2 0 0 2 0 0 0 4)
