摘要
本实验建立了以反相HPLC同时测定人血清中慢心率及利多卡因的方法。以布比卡因为内标,流动相为酸性甲醇:水(65:35)内含?了胺0.11%,冰醋酸0.065%(PH7.46),紫外220nm检测。慢心率回收率为98.48—104.99%,日内和日间变异性(浓度各为5.010,1.804μg/ml)CV%分别为5.672 182.37 4.54(n=5);利多卡因回收率为99.13—103.79%,日内和日间变异性(逍度各为6.090 2.192μg/ml CV%分别为2.21 4.29 2.15 5.52(n=5)。将该法初步用于临床对数名患者进行血浓监测及时调整剂量,得到满意结果。
A simultaneous quantitative method was reported for the determinationof Mexiletine and lidocaine inhuman plasma byreversed phase HPLC. Bupivacaine was used as an internal stanhard. The mobile phase wasconsisted of 65% changer Methyl Alcohol and 35% water, modified with 0.11% n-Bulylamine and 0.065% Glacial Acetic Acid (pH7.46). The detection was carried out at uvzzonm. The recoveries of Mexile-tine were 98.48～104.99%. The cv of within-day and between-day (The concentration was 5.0101.804ug/ml) (n=5) were 5.67, 2.18, 2.37, 4.54, resptively. The recoveries of Lidocaine were99.13--103.79%. The cv of within-day and between-days (The concentration was 6.090 2.192ug/ml) (n=5) were 2.21, 4.29, 2.15, 5.52 respetively. The method was used initialy for numbers of patients for adjusting the dose. A satisfactory result wasobtained for clinical therapentic drug monitoring.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
1992年第5期211-213,共3页
Chinese Journal of Hospital Pharmacy
关键词
慢心率
利多卡因
血药测定
HPLC
Mexiletine
Lidocaine
Blood drug monitoring
