摘要
利用原代培养大鼠肝细胞的方法,研究右旋儿茶素(d-CTC)对四氯化碳(CCl_4)或半乳糖胺(d-GalN)引起肝细胞毒性的作用,并观察该药对肝酶的直接作用。结果表明:d-CTC 0.6～5.0mg/ml可使含10mmol/L CCl_4或5mmol/Ld-GalN肝细胞培养液中的GOT.GPT及LDH活性显著降低,并呈量效关系。d-CTC 2.5～5.0mg/ml可直接抑制LDH及GPT活性,但对GOT无明显影响。其抑酶作用与抗肝毒而降低酶活性比较,有非常显著的差别,提示d-CTC具有较强的抗肝毒作用。
The effects of d-catechin (d-CTC) on carbon tetrachloride- and d-galacto-samine (d-GalN)-induced cytotoxicity in primary cultured rat hepatocytes were examined.After 1.5 h preincubation, d-CTC was added at doses of 0.1-5.0mg /ml to culture medium together with 10 mmol/L CCU or 5 mmol/L d-GalN, respectively. GOT, GPT and LDH levels were measured 1.5 h after treatment. The results showed that d-CTC at doses of 0.6 to 5.0 mg/ml could protect the hepatocytes against the toxic effects of CCU and d-GalN. At the higher doses (2.5-5.0 mg/ml), d-CTC showed weak inhibition of LDH and GPT activities, but these did not influence its antihepatotoxic activity.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1992年第3期194-200,共7页
Acta Academiae Medicinae Sinicae
基金
国家自然科学基金
关键词
右旋儿茶素
四氯化碳
肝细胞
d-catechin carbon tetrachloride d-galactosamine hepatocytes
