摘要
人间期核内的Y小体是七十年代初期细胞遗传学上的一项重要发现.Pearson等首先报道正常男性口腔粘膜细胞的间期核以喹吖因染色,可观察到1个约0.25μm的荧光点。此后,其他学者以类似方法也相继在人多种细胞间期核内发现该荧光点。1971年巴黎人类细胞遗传学标准会议将男性间期核内的这种荧光点命名为Y染色质或Y小体。Y小体的数目和大小分别同Y染色体的数目及其长臂末端能被喹吖因类着色的异染色质的数量一致,根据这一原理,国内外众多实验室建立了Y小体显示法。
A modified staining method for Y chromatin is reported here which proved to be rapid and efficient for sex differention diagnosis. Samples were fixed in methanol: acetic acid (3:1) solution and stained with dyes in McIlvaine buffer. Y chromatin in buccal smears from normal males could be clearly revealed by this method. With this modified method and X-chromatin examination, one testicular feminization was found during examination of 1342 atheletes for the 11th Asian Games.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1992年第3期229-232,共4页
Acta Academiae Medicinae Sinicae
