摘要
淋病是我国近期流行的主要性传播疾病之一,常规进行淋球菌的分离培养以进行诊断,但费时费事。本研究利用金黄色葡萄球菌A蛋白(SPA)具有吸附IgG的Fc段的特性,建立了淋球菌的协同凝集(COA)试验。该法用于临床标本的检测和淋球菌菌株的鉴定,被证明为一种快速的鉴定淋球菌和诊断淋病的方法。
Gonococcal coagglutination assay reagent was produced on the basis of a SPA absorbent of specific antibody against Neisseria gonorrhoeae reference strains. Using this reagent, the assay could detect a minimum bacterial concentration of 10~6 CFU/ml. No crossreactivity was found with bacteria such as Escherichia coli, Staphylococcus aureus, Branhamella catarrhalis, Streptococcus faecalis, Pseudomonas aeruginosa and Neisseria meningitidis. Fifty-five Neisseria gonorrhoeae strains isolated from patient discharges in 1989 were screened in parallel wing Phadebact monoclonal coagglutination assay reagent and our reagent, the resulting correspondence rate was 100％. We examined 71 suspected gonorrhoea patients by detecting urethral discharges and cervical secretions with our reagent and compared the results with those of the standard culture method: the results were equivalent. It is concluded that this reagent can be used for the rapid identification of N. gonorrhoeae and for the rapid diagnosis of acute infective patients.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1992年第2期137-140,共4页
Acta Academiae Medicinae Sinicae
基金
国家自然科学基金资助课题
