摘要
目的 体外研究改良喷砂表面对成骨细胞功能、分化的影响 ,从细胞水平揭示该表面促进骨愈合的作用机制。方法 成骨细胞为原代分离、培养的第 5代人胚成骨细胞 ,选择细胞层碱性磷酸酶 (alkalinephosphatase,ALP)活性、蛋白质含量以及培养液中骨钙素含量作为评价成骨细胞功能、分化的 3项指标。采用酶动力法检测ALP活性、采用考马斯亮蓝法测定蛋白质含量、采用放免测定技术检测骨钙素含量 ,所得各值进行标准化处理。实验分 3组 :改良喷砂表面组、光滑表面组和细胞培养板空白对照组 ,细胞培养取 4d和 1 3d两个时间点。结果 细胞培养 4d ,改良喷砂表面组细胞层ALP活性 [(1 7 390± 1 59)nmolPNP·min- 1 ·1 0 - 6 个细胞 ]、蛋白质含量 [(1 52 7± 1 6 3) μg/1 0 6个细胞 ]以及培养液中骨钙素含量 [(43 0± 6 1 )ng/1 0 6 个细胞 ]均明显高于光滑表面组 [分别为(1 0 978± 1 879)nmolPNP·min- 1 ·1 0 - 6 个细胞、(58 0± 5 9) μg/1 0 6 个细胞及 (2 4 9± 6 0 )ng/1 0 6 个细胞 ]。培养至 1 3d ,各项指标两组间差异消失。结论 从细胞学角度证明改良喷砂表面具有加快种植体骨愈合的作用 。
Objective To study effects of the modified sandblasted surface of titanium implants, developed by authors, on the bone healing process Methods Osteoblasts were derived from the 5th passage of human fetal osteoblasts after primary isolation Alkaline phosphatase (ALP) activities and protein contents of cellular layers, and osteocalcin contents in culturing medium were employed as criteria to evaluate osteogenic functions and differentiation of osteoblasts The ALP activity was assayed utilizing the kinetic method, the protein content utilizing the Coomassie′s method, and the osteocalcin content utilizing the radioimmune assay (RIA) method Values of all criteria were divided by the corresponding cell numbers of different groups at a respective time point for the purpose of standardization Samples were assigned to three groups the modified sandblasted surface group, the smooth surface group and the blank control group, The culture was ended at, 4 days and 13 days Results At 4 days of culture, the modified sandblasted surface group showed a superiority to the smooth group with respects to the ALP activity [(17 390±1 595)nmol PNP·min -1 ·10 -6 cells vs (10 978±1 879)nmol PNP·min -1 ·10 -6 cells] and protein content [(152 7±16 3)μg/10 6 cells vs (58 0±5 9)μg/10 6 cells] of cellular layers and the osteocalcincontent [(43 0±6 1)ng/10 6 cells vs (24 9±6 0)ng/10 6 cells] in culturing medium Till the 13th day of culture, no differences were detected Conclusions It is cytologically proved that the modified sandblasted surface can accelerate the bone healing process of implants though the improvement of osteoblastic functions and differentiation
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2003年第4期254-256,I002,共4页
Chinese Journal of Stomatology
