摘要
应用EB病毒转化技术,由人脐带血建立了3株淋巴母细胞株(CKM-c,CKM-1和CKM-8)。经1年余连续传代120次,细胞均保持良好的生长能力。3株细胞平均倍增时间为24.5～36h,电镜检查符合B淋巴细胞特征;EBNA、VCA-IgA,MA和EA4种EBV抗原均为阴性;染色体众数为48～50条。CKM-c在新城鸡瘟病毒诱导下,能产生高效价干扰素。CKM-8经6-硫代鸟嘌呤(6-TG)和乌苯苷双耐药培养,现已能耐受30μg/ml 6-TG和10^(-5)mol/L乌苯苷,成功地建立了1株对HAT敏感,HGPRTase缺损的双耐药淋巴母细胞,该细胞可作为亲本细胞,用于人-人杂交瘤研究工作。
Three lymphoblastoid cell lines (CKM-c, CKM-1 and CKM-8) have been established by EBV-transformation from cord blood. They have been maintained for 1 year after 120 passages with rapid growth. The population doublingtimes were 24.5h (CKM-1), 30h(CKM-8)and 36 h (CKM-c).The B lymphocyte features were identified under an electron microscope. EBV antigens including EBNA, VCA-Ig A, MA and EA were negative in all the cell lines. The median chromosome number was 48～50. Interferon (IFN) was induced by NDV treatment of the CKM-c cell line. CKM-8 has been modified to be resistant to both thioguanine (30μg/ml) and Ouabain (10^(-5)) for the production of humanhuman hybridomas.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1992年第1期63-67,共5页
Acta Academiae Medicinae Sinicae
