摘要
目的 探讨白细胞介素 18(IL 18)在系统性红斑狼疮 (SLE)发病中的作用。方法 采用逆转录多聚酶链反应 (RT PCR)及酶联免疫吸附法 (ELISA)测定 40例SLE患者及 2 0例正常对照者血浆中IL 18分泌水平及外周血单一核细胞 (PBMC)IL 18mRNA表达量。结果 SLE患者血浆IL 18水平及PBMCIL 18mRNA的相对表达水平与SLEDAI呈正相关。活动期SLE患者血浆IL 18水平及PBMCIL 18mRNA表达水平显著高于非活动期SLE患者及正常对照 (P均 <0 .0 1)。结论 外周血IL 18可能并未参与SLE的早期发病过程 ,但当疾病发展到一定程度 ,IL 18分泌水平升高 ,可能在疾病的进一步发展中起一定的作用。
Objective To investigate the role of interleukin 18(IL-18) in patients with systemic lupus erythematosus(SLE).Methods The level of plasma IL-18 and the expression of IL-18mRNA of peripheral blood mononuclear cells(PBMC) were evaluated by enzyme-linked immunosorbent assay(ELISA) and reverse transcriptase polymerase chain reaction(RT-PCR) respectively in 40 patients with SLE and 20 normal controls.Results The level of plasma IL-18 and the expression of IL-18mRNA of PBMC were related positively with SLE DAI,and increased significantly in the patients with active SLE,compared with inactive SLE patients and normal controls,but there was no difference statistically between inactive SLE patients and normal controls.Conclusion Peripheral blood IL-18 may not be involved in onset procedure of SLE,but high plasma IL-18 level may play a role in the progress of SLE.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2004年第4期208-209,共2页
The Chinese Journal of Dermatovenereology
基金
广东省自然科学基金资助项目 (0 0 1 0 82 )
