摘要
噬菌体展示技术是一种目的基因在噬菌体表面的表达产物与亲和选择相结合的技术。噬菌体结构蛋白pⅢ和pⅧ均可作为载体来展示外源多肽或蛋白质。初期阶段 ,噬菌体展示技术主要是用于单链可变区抗体、随机多肽库等的筛选 ,但近年来随着噬菌体cDNA表达型文库的构建 ,噬菌体展示技术的用途逐渐扩大。目前噬菌体展示技术不仅可以用于研究蛋白结合蛋白 ,而且还可以用来研究DNA/RNA结合蛋白 ,以及非蛋白小分子物质的结合蛋白等。因为蛋白 蛋白之间的结合是许多生物学效应的基础 ,因而噬菌体展示技术的应用范围进一步得到拓展。噬菌体展示技术在研究自身抗体结合与识别的自身抗原、信号转导过程中蛋白之间的相互作用以及肝炎病毒基因表达调节方面都有十分重要的应用前景。
Phage display is a technique that combines the expressed protein/peptide on the surface of a bacteriophage and its affinity binding capacity. The pⅢ and pⅧ genes are both suitable target sites for insertion of foreign cDNA fragment. In the early stage of this technique, it was mainly used for screening and identification of single chain antibody(scFv) and/or random peptide. Along with the construction and application of cDNA library for phage display, the application of this technique has been significantly broadened. At present phage display is one of the most important and efficient techniques for the study of protein-protein interaction, and DNA/RNA-binding proteins. Even non-protein small molecule-binding proteins could be defined by this technique. Recent progress has demonstrated that the phage display technique is a powerful tool for the investigation of autoantigen, tumor marker, proteins related to signal transduction, and even regulatory proteins involved in the gene expression of hepatitis viruses.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2004年第1期4-7,共4页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金 (编号C39970 674
C0 30 1 1 4 0 2 )
军队"九五"科技攻关项目 (编号 98D0 63)
军队留学归国人员启动基金 (编号 98H0 38)资助课题
