摘要
嗜肺军团杆菌(LP)所引起的肺炎病死率高,易暴发流行,早期病原诊断困难。本研究应用聚合酶链式反应技术对嗜肺军团杆菌血清1、3、5型中巨噬细胞感染潜能基因(mip基因)的部分编码区域进行特异性扩增,经琼脂糖凝胶电泳、溴乙锭染色检测扩增产物。结果3型LP均出现650bp特异性阳性条带。检测灵敏度为80ng,本研究结果对嗜肺军团杆菌肺炎的早期诊断、及时治疗、降低病死率及控制暴发流行有重要意义。
The pneumonia caused by legionella preumophilla(LP) is serious dise ase with high fatality rate. Strains of LP, (serogroap 1, 3, 5)were deteoted based upen amplification of a portion of the coding region region of the macrophuge infectivity potentiator gene using polymerase chain reaction(PCR). PCR-amplified DNAs were detected by using agarose gel electrophoresis stained in ethidium bromide solution. Bands of 650 bp positive amplified DNA of the three serogroups of LP were detected in 80 ng level with PCR. Application of the technique might be helpful in the early diagnosis and control of fulminant epidemic.
出处
《中国医科大学学报》
CAS
CSCD
1992年第6期432-435,共4页
Journal of China Medical University
基金
辽宁省科委资助项目
关键词
聚合酶链式反应
嗜肺军团杆菌
肺炎
polymerase chain reaction
legionella pneumo, hilla
early diagnosis
