摘要
用酶联免疫检测方法对通过Ri质粒遗传转化药用植物甘草后所获得的毛状根中之内源生长调节物质进行分析,结果在不添加任何植物生长调节物质的1/2 MS培养基上生长迅速的甘草毛状根中IAA、ZRs、iPAs的含量大大富于在同样培养条件下生长缓慢的甘草实生苗根中的含量,说明内源生长素、细胞分裂素类物质的高含量是甘草毛状根生长迅速的原因。
This paper reports the experiment results about the levels of endo-plant hormones in hairy-roots obtained from transgenic Glycyrrhiza uralensis by Ri plasmid with ELISA: the contents of IAA、ZRs、iPAs of hairy-roots of fast growth G. uralensis in 1/2 MS medium without adding plant-growth-regulators are higher than those of slow growth roots of seedling from seeds of G. uralensis in the same culture condition. It is shown that high contents of endo-auxins and cytokinins cause the fast growing of hairyroots of G. uralensis.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1992年第6期340-343,共4页
Journal of China Pharmaceutical University
关键词
甘草
毛状根
植物
生长调节物质
Hairy roots of G. uralensis
Plant growth regulators
IAA
ZRs(Zeatin riboside)
iPAs(isopentenyladenosines)
