摘要
目的 运用表达序列标签 (expressedsequencetag,EST)技术 ,从日本血吸虫成虫cDNA文库中分离、鉴定和分析表达基因序列 ,为日本血吸虫病的防治提供候选疫苗和药物靶点。方法 构建日本血吸虫成虫cDNA文库 ,随机挑取重组阳性克隆进行测序 ,将获取的EST登录GenBank ,并进行生物信息学分析。结果 随机挑取 382个阳性克隆进行测序 ,获得 1 49个EST(登录号分别为 :CA747382～CA747391 ,BU91 70 5 5～BU91 70 5 8,CA30 5 30 7～CA30 5 30 9,BU5 81 980 ,BU5 82 4 0 0～BU5 82 4 0 3,BU66690 6和CA9465 4 8～CA946666) ,同源性比较发现 ,部分序列与血吸虫的生活史的不同阶段和不同性别序列有一定的同源性。结论 EST技术有助于快速、经济地获取和研究日本血吸虫表达基因。
Objective To acquire and analyze adult stage Schistosoma japonicum (Chinese strain) expressed sequence tags (EST) from an adult Schistosoma japonicum cDNA library and to search new vaccine candidates and drug targets for preventing, controlling and treating schistosomiasis. Methods A cDNA library was constructed from Shistosoma japonicum (Chinese strain) adult stage. Positive clones were selected randomly from the cDNA library and were sequenced. After analyzing in GenBank databases by BLAST programs and other programs ESTs were acquired and submitted to GenBank. Then they were given accession numbers. Results 149 ESTs were acquired from a total 382 clones that were randomly selected from the adult Schistosoma japonicum cDNA library. All ESTs were successfully submitted to the dbEST at Genbank. ESTs accession numbers were CA747382～CA747391, BU917055～BU917058, CA305307～CA305309, BU581980, BU582400～BU582403, BU666906 and CA946548～ CA946666. Some of ESTs were homologous with the sequences of male, female, egg, cercariamiracidia, and schistosomula of Schistosoma japonicum. Conclusions EST strategy is a rapid, efficient and economical method to acquire the ESTs of the adult stage Schistosoma japonicum (Chinese strain) from cDNA libraries.
出处
《南华大学学报（医学版）》
2004年第1期8-12,共5页
Journal of Nanhua University(Medical Edition)
基金
湖南省卫生厅重点项目基金的资助 (NO .2 0 0 1-Z0 5 )
