摘要
本实验研究了大白鼠肠系膜淋巴结移植后的结构、功能以及细胞学的变化。同时观察了白细胞间介素2(Il-2)和棒状杆菌(Corynebacterium Parum C.P.)对移植后淋巴结再生的影响。结果表明淋巴结移植后体积逐渐缩小,细胞成份不断减少,丧失了正常的组织结构和功能。而淋巴结移植后其输入淋巴管再生的失败是导致其退化的主要原因。IL-2可能具有调节淋巴结内再循环淋巴细胞的作用而有利于维持移植后淋巴结的完整性。
The cellular, architectural and functional changes of transplanted mesenteric lymph nodes in the rats were studied.After the lymph nodes were transplanted with interrupting both of afferent and efferent lymphatics, the nodes gradually depleted the celluar content. One month after operation the recirculating lymphoeytes in the transplanted node were 3.5% of that in the host nodes, while the number of cells of the transplanted node was only 28%. The number of macrophages also decreased obviously. In paracortex, the cells of high endothelial venule (HEV) became flattened and HEV disappeared gradually. In the cortex, the germinal centers and follicles also diminished considerably in number and disappeared completely in 3 month. It was difficult to distinguish the normal compartments in the operated nodes 3 months after transplantation. No regenerated afferent lymphatics were identified in the transplanted nodes. Lacking of antigen and stimulated lymphocytes brought histological and functional involution of the transplanted lymph nodes.The effect of Corynebacterium Parvum (C.P) and interleukin 2 (IL-2) on the regeneration of the transplanted lymph node was observed. The injection of C. P (3mg/week) subcutaneously in the skin of antieipated lymph drainage region showed noinfluence on the lymph node and lymphatics. The grafted node underwent a progress ofinvolution. The injection of IL-2 (500u twice a week ) brought about more recirculating lymphocytes in the node, although the immunological function remained absent in the node. This effect may help to the maintenance of the intergrity of transplanted lymph node.
出处
《中国修复重建外科杂志》
CAS
CSCD
1992年第1期44-47,共4页
Chinese Journal of Reparative and Reconstructive Surgery
关键词
淋巴结移植
输入淋巴管
Transplanted lymph node Afferent lymphaties Structural and functional changes
