摘要
目的 评价抗 -H7血清琼脂方法对大肠埃希菌O15 7∶H7鞭毛抗原的检测效果。方法 采用抗 -H7血清琼脂法对来自人、动物、环境水和食品中的 10 9株疑似O15 7∶H7菌株进行H7鞭毛抗原鉴定 ,并设鞭毛抗原阳性和阴性对照菌株 ,同时与试管凝集法和H 7特异性基因聚合酶链反应 (PCR)法进行比较。结果 3种方法对鞭毛抗原阳性和阴性对照菌株的检测结果均成立 ,抗 -H7血清琼脂法与试管凝集法比较其敏感性、特异性和符合率均为 10 0 % ;而与H7特异性基因PCR法比较其敏感性、特异性和符合率分别为 93 .75 %、95 .3 1%和 97.2 5 %。结论 抗 -H7血清琼脂法是简便快速、稳定可靠的H7鞭毛抗原鉴定方法 ,具有推广使用价值。
Objective In order to evaluate anti-H7 serum agar medium assay for flagellar antigen of Enterohemorrhagic Escherichia coli O157∶H7.Methods 109 suspicious stains of O157∶H7 derived from feces of human,animal,food and water of enviroment were detected by anti-H7 serum agar medium assay for H7 flagellar antigen,in compare with tube agglutination test and the detection of species gene for H7, flagellor antigen positive and negative as control. Results The results showed that the three assay for detecting H7 flagellar antigen were good for the strains of positive and negative marker.The rate of sensitivity,specificity and coincident of anti-H7 serum agar medium assay were 100% at all in compare with tube agglutination test, and were 93.75%, 95.31%, 97.25% respectively in compare with detection of specific gene for H7. Conclusion The data showed that anti-H7 serum agar medium assay was a rapid,convenient and reliable method for detecting H7 flagellar antigen, so it was worthy to be used.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2004年第1期55-56,共2页
Chinese Journal of Vector Biology and Control
