摘要
采用无血清液体培养基、DMEM培养基和MEM培养基进行PK15细胞的培养,观察细胞增殖及传代效果,确定无血清液体培养基的营养功能。结果表明:无血清液体培养基只能维持第一代细胞的生长,不能用于第二代后的培养,效果不如DMEM、MEM培养基,且差异显著。
PK15 cells were cultured with free-blood serum media, DMEM media and MEM media in other to observe the effect of cell proliferation, cell transfer and define the ability of nutrition. The results showed that free-blood serum media only could maintain the growth of pre-generation cells, but not second-generation cells. It was not better than DMEM, MEM media.
出处
《黑龙江八一农垦大学学报》
2004年第1期62-64,共3页
journal of heilongjiang bayi agricultural university
关键词
培养基
PK15细胞
增殖
营养
猪传代肾细胞
PK15 cells
free-blood serum media
DMEM media
MEM media
cell proliferation
