摘要
观察了骨形态发生蛋白 4 (BMP4 )对原代分离培养的大鼠脑神经干细胞 (NSC)的胆碱能诱导分化效应。将从 2个月龄大鼠脑海马、纹状体等区域分离的细胞培养于含EGF和bFGF的DMEM/F1 2培养液中 ,在光镜下观察细胞的形态特征 ,并行神经巢蛋白 (nestin)细胞化学染色。 2 4h后 ,改换成含有BMP4的培养液 ,继续培养 7或 8天。在光镜下观察细胞的形态变化 ,并行胆碱乙酰转移酶 (cholineacetyltransferase ,ChAT)和nestin双标免疫细胞化学染色。结果表明 ,加BMP4继续培养 7或 8天后 ,光镜下见有 34%的培养细胞具有神经元的形态特征。用免疫细胞化学染色可见ChAT阳性的细胞与nestin阳性的细胞共存 ,其中ChAT阳性细胞占 2 8% ,nestin阳性细胞占 38%。总之 ,在培养液中加入BMP4 。
The cholinergic-inducing effect of BMP4 on isolated and cultivated rat′s cerebral neural stem cells(NSC) was examined. NSC isolated from two months old rat′s brain region like hippocampus and striatum was cultivated in a DMEM/F12 medium containing EGF and bFGF, and was identified with morphological character and nestin immunocytochemistry test.After 24 hours, cultivating the NSC with the BMP4-added medium for 7-8 days, then the microscopical change were observated, ChAT and nestin double-labelling immunocytochemistry test was done. Results showed that about 34% NSC of neuron-like character was observed by microscope in the paper.That ChAT-positive cells coexist with nestin-positive cells was found by immunocytochemistry test.There were 28% ChAT-positive cells and 38% nestin-positive cells in the study.Cholinergic neurons differentiated from NSC could be induced by adding BMP4 to the medium.
出处
《标记免疫分析与临床》
CAS
2004年第1期18-21,共4页
Labeled Immunoassays and Clinical Medicine
关键词
骨形态发生蛋白
神经干细胞
诱导
胆碱能
Bone morphogenetic protein
Neural stem cell
Induction
Cholinergic
