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用苯眯分离纯化类凝血酶

Separation and Purification of Thrombin-like Enzymes by Using Benzamidine
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摘要 用亲和层析分离纯化类凝血酶是一种迅速、高效的方法.根据酶与其竞争性抑制剂之间存在特异性相互作用的原理,本文制备了一种以类凝血酶竞争性抑制剂Benzamidine作为专一性配基的亲和吸附剂用于分离纯化类凝血酶.结果表明,用该吸附剂纯化重组类凝血酶能达SDS-PAGE电泳纯. An affinity adsorbent, Benzamidine-sepharose 4B, is used to separate and purify thrombin-like enzymes. The p-aminobenzamidine as a specific ligand is coupled to the matrix—Sepharose 4B. The recombinant thrombin-like enzyme—defibrase is used as a model in order to evaluate the efficiency of this biospecific affinity adsorbent. The homogeneity of the enzyme preparation is comfirmed as one band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
作者 龙姝 黄永华 杨青
机构地区 长沙理工大学化学与环境工程系 大连理工大学生物工程研究所
出处 《长沙电力学院学报（自然科学版）》 2004年第1期71-73,共3页 JOurnal of Changsha University of electric Power:Natural Science
关键词 亲和吸附剂 配基 苯眯啶 纯化 亲和层析 心血管疾病 蛇毒类凝血酶 苯眯 分离 affinity chromatography ligand benzamidine thrombin-like enzyme purification
分类号 R972 [医药卫生—药品] R914 [医药卫生—药物化学]
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长沙电力学院学报（自然科学版）
2004年 第1期

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